Hierarchically patterned polyurethane microgrooves featuring nanopillars or nanoholes for neurite elongation and alignment

• Lester Uy Vinzons,
• Guo-Chung Dong and
• Shu-Ping Lin

Beilstein J. Nanotechnol. 2023, 14, 1157–1168, doi:10.3762/bjnano.14.96

• microenvironment. While several of these focused on stem cell differentiation [9][10], a couple of studies explored their effects on axonal guidance. Lee et al. [11] found that nanorough microridges composed of laser-patterned Al/Al2O3 nanowires increase cell attachment and effectively guide dorsal root ganglia

Biomimetic chitosan with biocomposite nanomaterials for bone tissue repair and regeneration

• Se-Kwon Kim,
• Sesha Subramanian Murugan,
• Pandurang Appana Dalavi,
• Sebanti Gupta,
• Sukumaran Anil,
• Gi Hun Seong and
• Jayachandran Venkatesan

Beilstein J. Nanotechnol. 2022, 13, 1051–1067, doi:10.3762/bjnano.13.92

• bioactive glass has a mechanical tensile strength of 67 MPa. In addition, excellent biomineralisation was observed in SBF which shows the ability of bone mineral formation of chitosan nanocomposites [64]. Chitosan and tetraethoxysilane-based aerogels show cell attachment and proliferation of human

Bioselectivity of silk protein-based materials and their bio-inspired applications

• Hendrik Bargel,
• Vanessa T. Trossmann,
• Christoph Sommer and
• Thomas Scheibel

Beilstein J. Nanotechnol. 2022, 13, 902–921, doi:10.3762/bjnano.13.81

• , Universitätsstr. 30, 95440 Bayreuth, Germany 10.3762/bjnano.13.81 Abstract Adhesion to material surfaces is crucial for almost all organisms regarding subsequent biological responses. Mammalian cell attachment to a surrounding biological matrix is essential for maintaining their survival and function concerning

• obtained from fluorinated silica colloids, thin film deposition of silicone elastomers or nanoengineered superhydrophobic surfaces of Teflon®-coated aluminium [63][64][65]. Superhydrophobic surfaces have also been reported to be unfavorable for mammalian cell attachment and growth. This may be due to the

• ][153][154] or whole domains [155][156][157] and even proteins [158][159][160]. 3.2 Cell adhesive silk material surfaces Since the engineered ADF4 sequences lack cell binding sequences, biological interaction and cell attachment is quite limited to recombinant spider silk materials made thereof [161

Micro- and nanotechnology in biomedical engineering for cartilage tissue regeneration in osteoarthritis

• Zahra Nabizadeh,
• Mahmoud Nasrollahzadeh,
• Hamed Daemi,
• Mohamadreza Baghaban Eslaminejad,
• Ali Akbar Shabani,
• Mehdi Dadashpour,
• Majid Mirmohammadkhani and
• Davood Nasrabadi

Beilstein J. Nanotechnol. 2022, 13, 363–389, doi:10.3762/bjnano.13.31

• proliferation of chondrocytes to achieve unidirectional orientation was controllable by optimizing the size and spacing of VA–MWCNT micropillars. Cell attachment, proliferation, and ECM production reportedly were enhanced by micropillars in comparison to the control groups [137]. Apart from carbon nanotubes for

• , poly(propylene fumarate) (PPF) nanocomposites containing BNNTs showed increased mechanical reinforcement, higher adsorption of collagen I protein, excellent cell attachment as well as ECM deposition compared to the PPF control [144]. Halloysite clay, a natural aluminosilicate material, is an

• Biological cues in cartilage TE Natural ECM provides a desirable microenvironment containing biologically important motifs (Table 2) for cell attachment, spread, migration, proliferation, and differentiation [149]. This microenvironment is inevitably disregarded during the in vitro cell culture and expansion

Engineered titania nanomaterials in advanced clinical applications

• Padmavati Sahare,
• Paulina Govea Alvarez,
• Juan Manual Sanchez Yanez,
• Gabriel Luna-Bárcenas,
• Samik Chakraborty,
• Sujay Paul and
• Miriam Estevez

Beilstein J. Nanotechnol. 2022, 13, 201–218, doi:10.3762/bjnano.13.15

• improvement in the mechanical properties was observed after the incorporation of 5 wt of nano-TiO2 and a bone-like apatite structure was formed in simulated body fluid (SBF), which supported cell attachment and growth, showing the potential for bone TE applications [73]. Human gingival fibroblasts (HGFs) are

Multilayer capsules made of weak polyelectrolytes: a review on the preparation, functionalization and applications in drug delivery

• Varsha Sharma and
• Anandhakumar Sundaramurthy

Beilstein J. Nanotechnol. 2020, 11, 508–532, doi:10.3762/bjnano.11.41

• in multilayer films such as PGA/PLL, PAH/PAAm and hyaluronic acid (HA)/chitosan when one of the polymeric pair is grafted with an arginine–glycine–aspartic acid (RGD) peptide sequence [104][105][106]. Similarly, the PLL/PGA film showed better cell attachment when plain PGA was replaced with a laminin

Atomic force acoustic microscopy reveals the influence of substrate stiffness and topography on cell behavior

• Yan Liu,
• Li Li,
• Xing Chen,
• Ying Wang,
• Meng-Nan Liu,
• Jin Yan,
• Liang Cao,
• Lu Wang and
• Zuo-Bin Wang

Beilstein J. Nanotechnol. 2019, 10, 2329–2337, doi:10.3762/bjnano.10.223

• patterns (dots, holes or hexagons) [39]. Influences of stiffness and topography on L929 cell responses by AFAM imaging To further assess the fidelity of cell attachment to each substrate on the nanoscale, the L929 cells cultured on different substrates were imaged using AFAM. Figure 5 shows that the cells

Toxicity and safety study of silver and gold nanoparticles functionalized with cysteine and glutathione

• Barbara Pem,
• Igor M. Pongrac,
• Lea Ulm,
• Ivan Pavičić,
• Valerije Vrček,
• Darija Domazet Jurašin,
• Marija Ljubojević,
• Adela Krivohlavek and
• Ivana Vinković Vrček

Beilstein J. Nanotechnol. 2019, 10, 1802–1817, doi:10.3762/bjnano.10.175

• at 37 °C and 5% CO2 to allow cell attachment. The following day, AgNPs, AuNPs, AgNO3 and HAuCl4 were added to the wells in different concentrations and treated for 24 h. The MTT assay, based on the reduction of the yellow tetrazolium salt MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium

Biocompatible organic–inorganic hybrid materials based on nucleobases and titanium developed by molecular layer deposition

• Leva Momtazi,
• Henrik H. Sønsteby and
• Ola Nilsen

Beilstein J. Nanotechnol. 2019, 10, 399–411, doi:10.3762/bjnano.10.39

• of the same samples was measured after each period of water treatment. The three hour and four day time periods are the durations in which cells were cultured on these substrates for cell attachment and cell proliferation assays, respectively, in our previous study [23]. Unlike for the Ti-amino acids

Preparation of micro/nanopatterned gelatins crosslinked with genipin for biocompatible dental implants

• Reika Makita,
• Tsukasa Akasaka,
• Seiichi Tamagawa,
• Yasuhiro Yoshida,
• Saori Miyata,
• Hirofumi Miyaji and
• Tsutomu Sugaya

Beilstein J. Nanotechnol. 2018, 9, 1735–1754, doi:10.3762/bjnano.9.165

• the cells on groves were aligned along the direction of the grooves. In contrast, the cells on pillars and holes exhibited randomly elongated filopodia. The vinculin spots of the cells were observed on the top of ridges and pillars or the upper surface of holes. The results of a cell attachment assay

• showed that the number of surface-attached cells increased with increasing patterning of the gelatin surface. Unlike the cell attachment assay, the results of a cell proliferation assay showed that Saos-2 cells prefer grooves with diameters of approximately 2 µm and 1 µm and pillars with diameters of 1

• µm and heights of 500 nm. The number of cells on pillars with heights of 2 µm was larger than those of the other gelatin surface patterns tested. Conclusion: These data support that a detailed design of the gelatin surface pattern can control both cell attachment and proliferation of Saos-2 cells

Liquid-crystalline nanoarchitectures for tissue engineering

• Baeckkyoung Sung and
• Min-Ho Kim

Beilstein J. Nanotechnol. 2018, 9, 205–215, doi:10.3762/bjnano.9.22

• tissue-derived biopolymers used in pharmaceutics, cosmetics and food industries, have a strong advantage as a cell scaffold material in that they harbor specific amino acid sequences (such as Arg–Gly–Asp) on which cell attachment occurs through the binding of transmembrane receptors (such as integrin

Uptake and intracellular accumulation of diamond nanoparticles – a metabolic and cytotoxic study

• Antonín Brož,
• Lucie Bačáková,
• Pavla Štenclová,
• Alexander Kromka and
• Štěpán Potocký

Beilstein J. Nanotechnol. 2017, 8, 1649–1657, doi:10.3762/bjnano.8.165

• concentration-dependent manner. This was explained by the fact that MWCNTs at higher concentrations covered most of the bottom of the culture well and left only limited space for cell attachment and spreading [38]. Influence of particle type Figure 3 and Figure 4 show a comparison between the cell metabolic

Silicon microgrooves for contact guidance of human aortic endothelial cells

• Sara Fernández-Castillejo,
• Pilar Formentín,
• Úrsula Catalán,
• Josep Pallarès,
• Lluís F. Marsal and
• Rosa Solà

Beilstein J. Nanotechnol. 2017, 8, 675–681, doi:10.3762/bjnano.8.72

• , macroporous and micropillars, to study the effect of topography on the behaviour of endothelial cells. Collagen was found to stimulate cell adhesion and promote an enhanced cell attachment [32][33]. Herein, to mimic the elongated endothelium in natural lineal vessels, human aortic endothelial cells (HAECs

Fabrication of hybrid nanocomposite scaffolds by incorporating ligand-free hydroxyapatite nanoparticles into biodegradable polymer scaffolds and release studies

• Balazs Farkas,
• Marina Rodio,
• Ilaria Romano,
• Alberto Diaspro,
• Romuald Intartaglia and
• Szabolcs Beke

Beilstein J. Nanotechnol. 2015, 6, 2217–2223, doi:10.3762/bjnano.6.227

• of HA enhanced hydrophilicity and serum protein adsorption, and as a result, this increased pre-osteoblast cell attachment, spreading, and proliferation after four days of culture. Different technical routes have been explored for the synthesis of HA NPs, including mechanochemical synthesis [10

Tattoo ink nanoparticles in skin tissue and fibroblasts

• Colin A. Grant,
• Peter C. Twigg,
• Richard Baker and
• Desmond J. Tobin

Beilstein J. Nanotechnol. 2015, 6, 1183–1191, doi:10.3762/bjnano.6.120

• density of 1 × 104 cells/well. The plates were maintained overnight (16 h) in RPMI 1640 medium to allow for cell attachment. The plates were incubated for 24 h in serum-starved medium (i.e., lacking fetal bovine serum) to remove exogenous sources of growth factors, before being exposed to the tattoo ink

Oxygen-plasma-modified biomimetic nanofibrous scaffolds for enhanced compatibility of cardiovascular implants

• Anna Maria Pappa,
• Varvara Karagkiozaki,
• Silke Krol,
• Spyros Kassavetis,
• Dimitris Konstantinou,
• Charalampos Pitsalidis,
• Lazaros Tzounis,
• Nikos Pliatsikas and
• Stergios Logothetidis

Beilstein J. Nanotechnol. 2015, 6, 254–262, doi:10.3762/bjnano.6.24

• and its effect on cell attachment and growth was evaluated. The conditions of the surface modification were properly adjusted in order to define those conditions of the treatment that result in surfaces favorable for cell growth, while maintaining morphological integrity and mechanical behavior

• evaluate the influence of the plasma treatment on cell attachment, morphology and viability. Results and Discussion Morphological characterization of the plasma-treated scaffolds Electrospinning is a versatile and well-established technique broadly applied for the fabrication of nanofibrous meshes as

• nanofibrous scaffolds, cellular morphology and cell attachment were observed after 1, 3 and 7 days. Cells were fixed, after the predetermined time periods, in 4% formaldehyde/PBS, at rt for 20 min, permeabilized with PBS and incubated with methylene blue (blue fluorescence) at rt for 30 min. The cell surface

Release behaviour and toxicity evaluation of levodopa from carboxylated single-walled carbon nanotubes

• Julia M. Tan,
• Jhi Biau Foo,
• Sharida Fakurazi and
• Mohd Zobir Hussein

Beilstein J. Nanotechnol. 2015, 6, 243–253, doi:10.3762/bjnano.6.23

• cell attachment. Subsequently, the cells were treated with different concentrations of LD, SWCNT–COOH and SWCNT–LD for 24, 48 and 72 h. Following incubation, 20 µL MTT (5 mg/mL in PBS) was added to each well and the plate was incubated for 3 h. The excess MTT was then aspirated and the formazan

Coating with luminal gut-constituents alters adherence of nanoparticles to intestinal epithelial cells

• Heike Sinnecker,
• Katrin Ramaker and
• Andreas Frey

Beilstein J. Nanotechnol. 2014, 5, 2308–2315, doi:10.3762/bjnano.5.239

• . Conclusion: Luminal gut-constituents may both attenuate and augment the adherence of NPs to cell surfaces. These effects appear to be dependent on the particle size as well as on the type of interacting protein. While some proteins will rather passivate particles towards cell attachment, possibly by

Carbon-based smart nanomaterials in biomedicine and neuroengineering

• Antonina M. Monaco and
• Michele Giugliano

Beilstein J. Nanotechnol. 2014, 5, 1849–1863, doi:10.3762/bjnano.5.196

• have been studied by Ariano and co-workers [126] and by Thalhammer and colleagues [130], who observed that oxygen functionalisation [126] and deposition of a layer of 5–10 nm sized ND particles (NDPs) [130] (Figure 4a) improved cell attachment and did not compromise neuronal electrical activity. This